TABLE 5.
Minimum inhibitory concentration (MIC) of PHNX peptides (μg/ml) against multi-drug resistant and antibiotic susceptible strains of E. coli and S. aureus.
| Bacteria peptides | E. coli ATCC 51659 | S. aureus ATCC 33592 | E. coli ATCC 4157 | S. aureus ATCC BAA-1718 | Consensus from predictors (Table 4) |
| PHNX-1 | 32 | 64 | 16 | 32 | Active (probability ≥ 0.92) |
| PHNX-2 | >100 | >100 | >100 | >100 | Mixed predictions (see text, 0.54 by CAMPR3-RF) |
| PHNX-3 | >100 | >100 | >100 | >100 | Mixed predictions (see text, 0.73 by CAMPR3-RF) |
| PHNX-4 | >100 | >100 | >100 | >100 | Active (except by DBAASP) |
| PHNX-5 | >100 | >100 | >100 | >100 | Active (except by DBAASP) |
| PHNX-6 | >100 | >100 | >100 | >100 | Mixed predictions (0.70 RF-AmPEP) |
| PHNX-7 | >64 | >100 | >100 | >100 | Active (except by CLASSAMP-SVM) |
| PHNX-8 | 64 | >100 | 32 | >100 | Active (except by CLASSAMP-SVM) |
| LL-37 | 32 | >64 | NT | NT | |
| BF-CATH | 8 | 64 | 4 | NT | |
| IDR-1018 | 16 | 16 | NT | 16 |
NT refers to not tested. LL-37, BF-CATH, and IDR-1018 were control peptides tested against the bacterial strains. The consensus from the antimicrobial peptide predictors in Table 4 is indicated in the last column for comparison.