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In vitro osteogenesis of hMSCs seeded on each MAHS. (a) Time course changes in osteogenic gene levels in different MAHSs (including Runx2, ALP, OCN and Col-I) from days 7–42 were determined with real-time PCR. (b) Western blot for Runx2, ALP, Col-Ⅰ proteins. β-Actin protein (Actin) was used as control. All experiments were carried out in triplicate. n = 4 per group. Data are the mean ± s.d.
