Fig. 7.

(Ⅰ) Perfusion decellularization of whole heart scaffold. Retrograde perfusion of rat heart using polyethylene glycol (a), Triton X-100 (b) or sodium dodecyl sulfate (SDS) (c) over 12 h. Corresponding H&E staining of thin sections from LV of rat hearts perfused with polyethylene glycol (d) or Triton X-100 (e). (f) H&E staining of a thin section of SDS-treated heart showing no intact cells or nuclei. Reproduced from Ref. [14]. (Ⅱ) Morphology of (a) native myocardium and (b) decellularized myocardial scaffold after decellularization treatment of 2.5 weeks. (Ⅲ) Mason's trichrome staining for (a) native myocardium and (b) decellularized myocardial scaffold.H&E staining of (c) the longitudinal and transversal views of the acellular scaffold, and (d) vasculature templates in the decellularized myocardial scaffold. Reproduced from Ref. [295].