Fig. 8.

dECM for lung, liver and kidney tissue engineering. (Ⅰ) Perfusion decellularization of whole rat lungs. (a) Freshly isolated lung (left), after 60 min of SDS perfusion (middle), and after 120 min of SDS perfusion (right). (b,c) Corresponding Movat's pentachrome staining of thin sections from parenchyma of native (left panels) and decellularized (right panels) lung. (d) Corresponding Verhoeff's elastic-tissue staining of thin sections from parenchyma of native (left) and decellularized (right) lung. (e) TEM image of decellularized lung. Reproduced from Ref. [15]. (II) DLM retains intact lobular structure and vascular bed. (a) Representative photograph of decellularized left lateral and median lobes of rat liver. (b) The vascular tree, after perfusion with Allura Red AC dye. (c,d) Corrosion cast model of left lobe of a normal liver (c) and the DLM (d). (e–g) SEM images of a vessel (e), a section featuring bile duct-like small vessels (arrows) (f), ECM within the parenchyma (g), with hepatocyte-size free spaces. Reproduced from Ref. [17]. (III) Perfusion decellularization of whole rat kidneys. (a) Freshly isolated kidney (left), after 6 h of SDS perfusion (middle), and after 12 h of SDS perfusion (right). (b) Corresponding Movat's pentachrome stained sections of rat kidney during perfusion decellularization. (c) Representative immunohistochemical stains of cadaveric rat kidney sections showing distribution of elastin, collagen IV and laminin. (d) Corresponding sections of decellularized rat kidney tissue after immunohistochemical staining for elastin, collagen IV and laminin. (e) TEM of a cadaveric rat glomerulus showing capillaries (C), mesangial matrix (M) and podocytes (P) surrounded by Bowman's capsule (BC). (f) TEM of decellularized rat glomerulus exhibiting cellularity in decellularized kidneys. (g, h) Biochemical quantification of DNA and total collagen in cadaveric and decellularized rat kidney tissue. Reproduced from Ref. [18].