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The multiplexed ctDNA detection platform using 3D-coded ID-rings. A, B. Schematic illustration of the principle of the 3D-coded ID ring platform. C. 3D scatter plots of mixed codes in the presence of KRAS, NRAS, PIK3CA, BRAF, and a mixture of the four ctDNAs as indicated. D. 3D bar plot of the mean fluorescence intensities under indicated conditions. Each column bar represents the average value of three independent assays.
