Figure 4.

Tumor cell sensitivity is not dependent on the potential to trigger NK cell activation. (A) LogRank test p-values for MCA-tumors 948, 970, 972, 969 and 943. (B) IFN-γ production and CD107a expression by NK cells after 4 hours of coculture with the indicated target cells. Pooled results of at least 2 experiments per tumor cell line with each dot representing one biological replicate. (C) Cytotoxicity assays assessing ⁵¹Cr release after 4 hours of coculture of NK cells and the indicated target cells at the indicated ratios. Pooled results of at least 2 experiments per tumor cell line with at least 2 biological replicates per experiment. (D) Details of the cytotoxicity assays of (panel C) assessing ⁵¹Cr release after 4 hours of coculture of NK cells and the indicated target cells at 4:1 ratio. Pooled results of at least 2 experiments per tumor cell line with each dot representing one biological replicate.(mean ± SD; Student’s t tests (comparison with no stimulation (B) or RMA (C) conditions); *p < 0.05; **p < 0.01; ***p < 0.005; ns, not significant).