Figure 4.

Gating strategy for flow cytometry analyses of CD4⁺ and CD8⁺ T cells after BNT162b vaccination. (A) Identification of activation induced markers (AIM⁺ cells). Briefly, “living CD3⁺ T cells” are identified as 7-aminoactinomycine D (7AAD)-negative and CD3-positive cells. Among this population, CD4⁺ and CD8⁺ T cells are selected according to CD4⁺ and CD8⁺ expression, respectively. AIM⁺ cells among CD4⁺ T cells are both CD154⁺ and CD69⁺. AIM⁺ cells among CD8⁺ T cells are both CD107a⁺ and CD69⁺. (B) Representative plots displaying IFNγ, IL-2, and TNFα expression among AIM⁺CD4⁺ and CD8⁺ T cells.