Table 1.
The genes information of iron transporter and matrix proteins in uterus.
| Genes | Primer sequence (5’-3’) | Annealing temperature (°C) |
|---|---|---|
| β-actin | Forward: AATGGCTCCGGTATGTGCAA Reverse: GGCCCATACCAACCATCACA |
60 |
| CALB1 | Forward: TAATGCTCAGGATGTTTGAT Reverse: ATGCTTTTCTTGTATGTCGC |
60 |
| ATP2A2 | Forward: CTATTGGATGTTATGTTGGT Reverse: ATGACAGGTAGTGAGATTTT |
60 |
| CA2 | Forward: AAACTTTGACCCTACTGGAC Reverse: CTCTGACTTCTCTGCTCTTC |
60 |
| SLC4A4 | Forward: CAGACCAAGAAGTCCAACCT Reverse: GTCAGAGCACCCAACATAAC |
60 |
| SLC4A5 | Forward: AGCACCAAACACCACTAACG Reverse: ATCCACACCACAAAACAGCA |
60 |
| SLC4A7 | Forward: TGGTTGGTGTTATGTTGGGT Reverse: CAGGTCAGGTTGATGTTTGG |
60 |
| SLC26A9 | Forward: GGCGTGGGATTTTCCGTGTT Reverse: GCAGCTTCGTTGGTGGTTGC |
60 |
| ATP6V1B2 | Forward: CCAACCATTGAACGCATTAT Reverse: GTGCAGCTGTCTGTCCACAT |
60 |
| ATP6V1C2 | Forward: CTCTGAGTACCTCATCACGC Reverse: GCTCTTCCTTTTCACATTTC |
60 |
| SPP1 (OPN) | Forward: CAGTTCTTTGCTTATGCCTTATCAG Reverse: GCCAGGTCATTCTGCGGGT |
60 |
| OC-116 | Forward: GAGGAACCAGACGCAGATAAAGAAG Reverse: GTTTTCAGGCTTGGGGCTGTA |
60 |
CALB1 (Calbindin D28K): Ca2+ intracellular transporter; ATP2A2 (Endoplasmic reticulum calcium ATPase 2): Ca2+ ATPase; CA2 (Carbonic anhydrase 2): catalyze HCO3− formation; SLC4A4 (Solute carrier family 4 member 4): Na + /HCO3− cotransporter; SLC4A5 (Solute carrier family 4 member 5): Na + /HCO3− cotransporter; SLC4A7 (Solute carrier family 4 member 7): Na + /HCO3− cotransporter; SLC26A9 ((Solute carrier family 26 member 9): HCO3− /Cl − exchanger; ATP6V1B2 (Vacuolar H ATPase B subunit osteoclast isozyme): H+ pump; ATP6V1C2 (Vacuolar H ATPase B subunit osteoclast isozyme): H+ pump; SPP1(OPN, Osteopontin): mineralization inhibitor; OC-116 (Ovocleidin 116): core protein of dermatan sulfate proteoglycan.