Figure 1.

Development of the 10-color “MoT test” to concurrently analyze CD3⁺ T cells, CD4⁺ T_EM cells, CD279⁺ CD8⁺ T cells and CD14⁺ monocytes. (A) Antibodies against CD45, CD3, CD4 and CD8 were used as T cell backbone. Lineage antibodies against CD19, CD20, CD56 and CD66b were used for exclusion of B cells, NK cells and granulocytes. Antibodies against CD45RA and CCR7 were used for the characterization of memory T cell subsets. αCD279 was used to characterize late-activated and exhausted T cells. αCD14 and αCD16 were used for subsetting monocytes. (B–N) Titration of antibodies included in the MoT test. Optimal concentrations were chosen according to MFI and Stain Index.