Figure 2.

Gating strategy used for analysis of the MoT test.. Data were analyzed as follows: Selection of singlets; gating on CD45⁺ leukocytes; gating on CD3⁺ T cells and CD3^- cells; gating on Lineage-negative (Lin^-) cells within the CD3^- compartment; gating on CD14⁺ monocytes; gating on monocyte subpopulations defined by CD14 and CD16 expression; gating on CD4⁺ and CD8⁺ T cells within the CD3⁺ T cell compartment; gating on CD4⁺ memory T cell subsets; gating on CD279⁺ CD8⁺ T cells.