Figure 7.

Application of the MoT test to a melanoma patient cohort revealed sex- and stage-dependent differences in T cell and monocyte populations. Fresh whole blood EDTA samples from patients with stage II (n = 52), stage III (n = 21) or stage IV (n = 27) melanoma were investigated using the MoT test. No correlation (Pearson) with age was found for CD3⁺ T cell % (A), CD4⁺ T_EM cell % (B), CD279⁺ CD8⁺ T cell % (C) and CD14⁺ monocytes % (D). No sex-dependent differences (unpaired t test) were found for CD3⁺ T cells (E) and CD4⁺ T_EM cells (F). CD279⁺ CD8⁺ T cells (G) and CD14⁺ monocytes (H) were increased in male patients (unpaired t test). (I) CD3⁺ T cells were decreased in stage IV patients (K.W. test). (J) CD4⁺ T_EM cells showed a significant stage-dependent increase (K.W. test). No stage-dependent differences (K.W. test) were found in CD279⁺ CD8⁺ T cells (K) and CD14⁺ monocytes (L).