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. 2021 Nov 18;8:784962. doi: 10.3389/fmolb.2021.784962

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Copyright © 2021 Aguilar Cosme, Gagui, Bryant and Claeyssens.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PDT treatment combinations lead to varying degrees of spheroid damage. PpIX and PpIX-CD show similar reductions to viability and spheroid area as light treatments (LT) were increased. Light fractionated treatments (2LT and 3LT) caused greater damage to spheroids, reaching around 25% viability and area in comparison to single light exposure (1LT). Each point represents a spheroid group with different PDT conditions (N = 3, n = 3). Similar combinations (e.g., PpIX 3LT 5 µg and 10 µg) could not be differentiated.