Skip to main content
. 2021 Nov 18;15:718087. doi: 10.3389/fncel.2021.718087

FIGURE 6.

FIGURE 6

Upregulated cytokine expression in βB1-CTGF retinae. (A) RT-qPCR analyses showed an upregulation of Infg mRNA levels in βB1-CTGF retinae at 5 (p = 0.009), but not at 10 and 15 weeks of age. (B) The mRNA expression levels of Cxcl1 were not altered in 5-week-old transgenic mice. At 10 and 15 weeks, significantly higher mRNA levels of Cxcl1 were noted in βB1-CTGF mice (10 weeks: p = 0.01; 15 weeks: p = 0.04). (C) RT-qPCR analyses revealed no changes in the mRNA expression levels of Cxcl2 in 5-week-old transgenic animals. A significant upregulation of Cxcl2 mRNA levels was noted in βB1-CTGF mice at 10 (p = 0.03) and 15 weeks (p = 0.04). (D) Cxcl10 mRNA levels were significantly upregulated in βB1-CTGF retinae at 5 (p = 0.03), 10 (p = 0.02), and 15 weeks (p = 0.002). (E) Representative pictures of retinal cross-sections labeled with an antibody against IP-10 (CXCL-10; red) and RBPMS (RGCs; green), while DAPI counterstained cell nuclei (blue). IP-10+ cells were predominantly observed in the ganglion cell layer and inner nuclear layer of βB1-CTGF mice. Values are median ± quartile ± minimum/maximum (n = 5–6/group). The dotted line in panels (A–D) represents the relative expression of the WT group. GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer. Scale bar: 20 μm. *p < 0.05, **p < 0.01.