TABLE 1.
Sequences of oligonucleotides.
| Gene | Forward (F) and reverse (R) oligonucleotides | GenBank acc. no. | Amplicon size | Primer efficiency |
| Actb-F Actb-R | ctaaggccaaccgtgaaag accagaggcatacagggaca | NM_007393.5 | 104 bp | 1.00 |
| C1qa-F C1qa-R | cgggtctcaaaggagagaga tcctttaaaacctcggatacca | NM_007572.2 | 71 bp | 1.00 |
| C1qb-F C1qb-R | aggcactccagggataaagg ggtcccctttctctccaaac | NM_009777.3 | 80 bp | 1.00 |
| C1qc-F C1qc-R | atggtcgttggacccagtt gagtggtagggccagaagaa | NM_007574.2 | 75 bp | 1.00 |
| C3-F C3-R | accttacctcggcaagtttct ttgtagagctgctggtcagg | NM_009778.3 | 75 bp | 1.00 |
| C5ar-F C5ar-R | gctgatggtgggttttgtgt gttcagcttctccaccctct | AY220494.1 | 229 bp | 0.94 |
| C5ar1-F C5ar1-R | gctgatggtgggttttgtgt gttcagcttctccaccctct | NM_001173550.1 | 229 bp | 0.85 |
| Cfb-F Cfb-R | ctcgaacctgcagatccac tcaaagtcctgcggtcgt | M57890.1 | 112 bp | 1.00 |
| Cfd-F Cfd-R | atggagtgacggatgacgac gggzgaggcactacactctg | NM_013459.4 | 109 bp | 1.00 |
| Cfh-F Cfh-R | aaaaaccaaagtgccgagac ggaggtgatgtctccattgtc | NM_009888.3 | 74 bp | 1.00 |
| Cxcl2-F Cxcl2-R | tgaactgcgctgtcaatgc gcttcagggtcaaggcaaac | NM_009140.2 | 153 bp | 1.00 |
| Cxcl1-F Cxcl1-R | agactccagccacactccaa tgacagcgcagctcattg | NM_008176.3 | 130 p | 1.00 |
| Cxcl10-F Cxcl10-R | gctgccgtcattttctgc tctcactggcccgtcat | NM_021274.2 | 110 bp | 1.00 |
| Hc-F Hc-R | tgacaccaggcttcagaaagt agttgcgcacagtcagctt | XM_017315669.2 | 69 bp | 1.00 |
| Infg-F Infg-R | atctggaggaactggcaaaa ttcaagacttcaaagagtctgagg | NM_008337.4 | 89 bp | 1.00 |
| Masp2-F Masp2-R | ggcggctactattgctcct aacacctggcctgaacaaag | NM_001003893.2 | 86 bp | 1.00 |
| Ppid-F Ppid-R | ttcttcataaccacaagtcaagacc tccacctccgtaccacatc | M60456.1 | 95 bp | 1.00 |
The listed oligonucleotide pairs were used in quantitative real-time PCR experiments, while Actb and Cyclophilin (Ppid) served as housekeeping genes. The predicted amplicon sizes are given. F, forward; R, reverse; acc. no., accession number; bp, base pair.