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. 2021 Nov 18;12:713713. doi: 10.3389/fmicb.2021.713713

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Copyright © 2021 Alves, de Oliveira, Franco-Luiz, Almeida, Gonçalves, Borges, Rocha, Rocha, Bezerra, Miranda, Capanema, Martins, Weber, Teixeira, Wallau and do Monte-Neto.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analytical sensitivity as revealed by the limit of detection (LoD). RNA was extracted from VTM-nasopharyngeal swab, and the genome viral copies input was calculated based on SARS-CoV-2 E gene-harboring plasmid (Bioclin #K228-1) calibration curve. RT-LAMP reaction was performed at 65°C during 30 min using WarmStart^® colorimetric master LAMP mix (NEB #M1800) in 20 μL final volume (upper panel). Amplicons were resolved in 2% agarose gel and stained with GelRed^® (Biotium #41003) to confirm DNA amplification (bottom panel). cps/μL, viral genome copies per microliter; NTC, nontemplate control; VTM, viral transport medium (Bioclin #G092-1).

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