Table 2.
Performance parameters of each in-house ELISA.
| Amine oxidase | Complement C5 | Serotransferrin | |
|---|---|---|---|
| Linear ranges | 6.25–400 ng/ml | 4.69–300 ng/ml | 0.76–8.67 mg/ml |
| LLOD | 3.13 ng/ml | 2.34 ng/ml | 0.51 mg/ml |
| Recovery rates | 80–120% | 80–120% | 80–120% |
| AUC-ROC (95% CI) | 0.8935 (0.7906–0.9964) | 0.9290 (0.8484–1.010) | 0.8580 (0.7291–0.9869) |
| Cut-off values | 192.0 ng/ml | 176.5 ng/ml | 2.1 mg/ml |
| Sensitivity % | 72.2% | 77.8% | 77.8% |
| Specificity % | 94.4% | 94.4% | 83.3% |
| Odds ratio | 13.00 | 14.00 | 4.67 |
| Positive predictive value | 93.8% | 94.3% | 84.6% |
| Negative predictive value | 74.3% | 78.4% | 76.2% |