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. 2021 Nov 18;11:781471. doi: 10.3389/fonc.2021.781471

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Copyright © 2021 Dong, Zhang, Liu, Li, Cheng, Li, Wang, Zheng, Dong and Zhang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LUZP1 is the direct target of miR-545-3p. (A) The binding sites of miR-545-3p and LUZP1 predicted by starBase. (B) The relative luciferase activity in T98G and LN229 cells was detected by dual-luciferase reporter assay. (C) The expression of LUZP1 in the public database (GEPIA). (D) The expression of LUZP1 in clinical samples detected by qRT-PCR. (E, F) The expression of LUZP1 in glioma cell lines detected by qRT-PCR and western blot. (G, H) LUZP1 expression in T98G and LN229 which transfected with miR-NC or miR-545-3p detected by qRT-PCR and western blot. (I, J) LUZP1 expression in T98G and LN229 cells transfected with vector, circ_0001367, circ_0001367+miR-NC or circ_0001367+miR-545-3p was detected by qRT-PCR and western blot. *P < 0.05, **P < 0.01.