Figure 4.

High-fat high-sucrose diet induces gut microbiota dysbiosis. (A) α-diversity measured by Chao index at baseline B for mice fed with normal chow (NC, N = 10) vs. high-fat high-sucrose diet (HFHS, N = 9). (B) Evolution of species richness measured by a fold change of Chao index at D0 relative to B. (C) PCoA of Bray-Curtis β-diversity index showing no NC-fed vs. HFHS-fed mice, at B and at D0. (D–H) Fold change of indicator OTU's abundances at D0 relative to B. (I, J) Short Chain Fatty Acids concentrations in μmol/g of caecal content or feces of NC-fed or HFHS-fed mice. Means +/– SEM are plotted. *p < 0.05, **p < 0.01 by ANOVA for parametric data or Kruskal-Wallis if not when more than 2 groups are compared, Mann-Whitney test or t-test for 2 groups comparison.