Figure 5.

Partially reversible effects of high-fat high-sucrose diet on the colonic transcriptome. (A) Experimental design. Mice were fed with a normal chow diet (NC, N = 10) or a high-fat high-sucrose diet (HFHS, N = 10) for 16 weeks or 8 weeks and then switched back to NC for 8 weeks (HFHS+NC, N = 10). (B) Hierarchical clustering heatmap of the most variable genes in NC vs. HFHS vs. HFHS+NC fed mice. (C–E) Expression levels of relevant genes in clusters C1 to C6 for groups of mice fed with NC, HFHS or HFHS+NC, mean expression values (log2) are plotted with standard deviations for up- and down-regulated genes. FDR * ≤ 0.05, ** < 0.01, *** < 0.001, **** < 0.001.