TABLE 1.
Antibody | Mean microtubule length (μm) ± SD | No. of microtubules/ centrosomeb
|
||
---|---|---|---|---|
<5 | 5–15 | >15 | ||
NRIgG | 9.0 ± 1.6 | 3 | 10 | 90 |
Anti-CPAP | 2.7 ± 0.5 | 37 | 82 | 5 |
Anti-γ-tubulin | 3.5 ± 0.5 | 7 | 72 | 24 |
The in vitro microtubule nucleation test from three independent experiments was performed as described in the text. Photographs were taken, and the length and number of microtubules were calculated for ∼100 asters.
Data indicate the number of centrosomas with <5, 5 to 15, or >15 microtubules per centrosome.