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. 2000 Oct;20(20):7813–7825. doi: 10.1128/mcb.20.20.7813-7825.2000

TABLE 1.

Effect of anti-CPAP and anti-γ-tubulin antibodies on microtubule nucleation in vitroa

Antibody Mean microtubule length (μm) ± SD No. of microtubules/ centrosomeb
<5 5–15 >15
NRIgG 9.0 ± 1.6 3 10 90
Anti-CPAP 2.7 ± 0.5 37 82 5
Anti-γ-tubulin 3.5 ± 0.5 7 72 24
a

The in vitro microtubule nucleation test from three independent experiments was performed as described in the text. Photographs were taken, and the length and number of microtubules were calculated for ∼100 asters. 

b

Data indicate the number of centrosomas with <5, 5 to 15, or >15 microtubules per centrosome.