Fig. 5.

Elevated FUBP1 activates the Wnt/β‐catenin signaling. (A) Western blotting analysis of Wnt/β‐catenin signaling in the indicated FUBP1‐transfected, FUBP1‐silenced, and vector control cells. (B) Western blotting analysis of β‐catenin in the nuclear fractions of the indicated cells. (C, D) Immunofluorescence staining (C) and quantification (D) of nuclear β‐catenin expression in the indicated cells. Scale bar, 50 μm. *P < 0.05. P values were determined by two‐tailed Student’s t‐test. (E) The mRNA levels of β‐catenin downstream genes by real‐time PCR in the indicated cells. *P < 0.05; **P < 0.01. P values were determined by two‐tailed Student’s t‐test. (F) The mRNA levels of key scaffold molecules in the upstream of β‐catenin genes by real‐time PCR in the indicated cells. *P < 0.05; ***P < 0.001. P values were determined by two‐tailed Student’s t‐test. (G) Western blotting analysis of DVL1 in the indicated cells. (H) Immunohistochemistry staining of DVL1 in 54 CRC specimens which collected from Sun Yat‐sen University Cancer Center. Two representative cases are shown. Scale bar, 1 mm. (I) DVL1 expression with FUBP1 expression in CRC specimens was determined by Pearson’s correlation analysis; ***P < 0.001. All bars represented the mean ± SD of three independent experiments.