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Immunocytochemistry and immunofluorescence analysis of spheroids from ovarian cancer patient ascites. Spheroid samples from 3 different OvCa patients were analyzed. (A) Paraffin‐embedded spheroids from two different patients (#5 and #16) showing strong EpCAM and E‐Cadherin expression and a few CD45+ cells. (B) IF staining of spheroids from patient #7 showing a similar pattern, namely a high percent of EpCAM+ and few CD45+ cells embedded in the aggregate structures.
