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. 2021 Jun 18;15(12):3578–3595. doi: 10.1002/1878-0261.13028

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© 2021 The Authors. Molecular Oncology published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Fig. 6 — Staining with mitochondrial markers. Ascites‐derived tumor spheroids and tumoroids from tumor tissue were stained with markers for mitochondrial mass and membrane potential using MitoTracker green FM and MitoTracker Red CMXRos, respectively. (A) Evaluation using flow cytometry showed high mitochondria mass levels in all samples and two subpopulations with different mitochondrial membrane potential. The subpopulation showing a higher mitochondrial membrane potential has been quantified in all samples including three matched pairs. (B) Confocal microscopy analyses (60×) ascites‐derived tumor spheroids and tumoroids from tumor tissue stained with MitoTracker green FM and MitoTracker Red CMXRos.