Fig. 2.

ADSC‐derived Cyr61 promotes CRC cell invasion and migration in vitro. (A) Representative images of transwell migration assays for HCT8 and DLD1 cells cocultured with culture medium alone (Med) or ADSCs in the presence or absence of 5 or 10 μg·mL⁻¹ anti‐Cyr61 antibody, or isotype‐matched IgG control (IgG). Scale bar = 100 μm, n = 3. (B) Representative images of wound‐healing assays for HCT116 and DLD1 cells cocultured with Med or ADSCs in the presence or absence of an anti‐Cyr61 antibody at 5 or 10 μg·mL⁻¹, or an IgG. n = 3 (C) Representative images of transwell migration assays for HCT8 and DLD1 cells with rCyr61 at different concentration. Scale bar = 100 μm, n = 3. (D) Representative images of wound‐healing assays for HCT116 and DLD1 cells with rCyr61 at different concentration. n = 3. Values are represented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, by one‐way ANOVA.