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. 2021 Jun 1;15(12):3447–3467. doi: 10.1002/1878-0261.12998

Fig. 3.

Fig. 3

Cyr61 binds to integrin αVβ5 on CRC cells. (A) Confocal microscopy for HCT8 cells in the presence or absence of rCyr61 at 40 ng·mL−1. Scale bar = 10 μm. (B) Immunoprecipitation of the membrane extracts of rCyr61‐treated HCT8 cells with anti‐Cyr61 antibody. (C) Western blot validation of mass spectrometric results with anti‐integrin αV, anti‐integrin β5, and anti‐Cyr61 antibody in immunoprecipitation products of the membrane extracts from rCyr61‐treated HCT8 and DLD1 cells with anti‐Cyr61 antibody. (D) Western blot analysis validation of the direct interaction between Cyr61 and integrin αVβ5. (E) Western blot analysis of integrin β5 expression in normal colonic cell lines and CRC cell lines. (F) Western blot analysis of integrin β5 expression in 10 CRC tissues and paired normal adjacent tissues. (G) IHC analysis of integrin β5 expression in the paraffin‐embedded CRC tissues and paired normal adjacent tissues. White scale bar = 200 μm. Black scale bar = 50 μm. (H, I) Kaplan–Meier analysis for OS and DFS of CRC patients with low or high expression of integrin β5.