TABLE 4.

Costs per sample for TB detection and susceptibility testing

Method	Detection cost ($)	Susceptibilitya cost ($)
		Two drugs	Four drugs
MODSb	0.77	1.72	1.80
MGITc	7.00	35.02	63.03
BACTECd	2.55	12.75	23.00
LJe	0.14	1.60	1.57
Microagar 7H11f	0.29	1.60	2.92
MABAg	1.23–2.43	5.62	6.87
PCRh	2.90
Acid-fast stain	0.10
Smeari

^aCalculated for two different concentrations of each drug. Two drugs, RIF and INH; four drugs, RIF, INH, ethambutol, and streptomycin. 

^bIncludes costs of Middlebrook 7H9 medium, glycerol, Bacto Casitone, OADC, PANTA, 24-well plate, Scotch polyethylene tape, pipette tips, and antimicrobial drugs. 

^cIncludes costs of MGIT (prepared tubes), OADC, PANTA, pipette tips, and antimicrobial drugs. 

^dIncludes costs of BACTEC vials and antimicrobial drugs; does not include the cost of syringes and reconstituting fluid. 

^eIncludes costs of LJ medium, glycerol, eggs, Scotch polyethylene tape, pipette tips, and antimicrobial drugs. 

^fIncludes costs of Middlebrook 7H11 medium, glycerol, OADC, cyclohexamide, Scotch polyethylene tape, pipette tips, and antimicrobial drugs. 

^gIncludes costs of Middlebrook 7H9 medium, glycerol, Bacto Casitone, OADC, PANTA, Tween-80, alamar blue reagent, 24- or 96-well plates, Scotch polyethylene tape, pipette tips, Combitips, and antimicrobial drugs. 

^hIncludes costs of one DNA extraction (Tris, EDTA, ethanol, 1.5-ml microcentrifuge tubes, and pipette tips), and two regular PCR reactions (Taq polymerase, 10× PCR buffer, deoxynucleoside triphosphates, MgCl, distilled water, 0.5-ml microcentrifuge tubes, pipette tips, primers, and agarose) and one nested PCR reaction (equal in cost to two regular PCR reactions). 

ⁱIncludes costs of microscope slides, auramine O or Ziehl-Neelsen stain, phenol, HCl, and ethanol.