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. 2021 Nov 18;9:766512. doi: 10.3389/fcell.2021.766512

FIGURE 4.

FIGURE 4

Effect of SIRT3 inhibitors on hair cells in the PTS group. (A) Schedule of 3-TYP and NE administration to C57BL/6 J mice. (B) ABR findings showing the effect of 3-TYP on the hearing threshold in mice after NE. (C) Immunohistochemical staining showing 3-TYP-induced changes in the number of hair cells (Myosin VII, green; DAPI, blue) after NE. (D) Quantification of Myosin VII and DAPI-positive HCs. (E) Immunohistochemical staining showing the effect of 3-TYP on the number of presynaptic (CtBP2, red) and postsynaptic (GluR2, green) structures after NE. (F) Quantification of CtBP2 and GluR2 overlapping fluorescent spots. (G) Quantification of CtBP2 or GluR2 fluorescent spots alone. (n = 10 per group). PTS, permanent threshold shift; NE, noise exposure; ABR, auditory brainstem response; DAPI, 4, 6-diamidino-2-phenylindole; OHC, outer hair cell, IHC, inner hair cell. NS, not significant; *p < 0.05; ***p < 0.001; ****p < 0.0001. Scale bars: C, 20 μm; E, 5 μm.