FIG. 2.

CD95L mRNA is induced following stimulation with anticancer drugs, and the induction is regulated on the transcriptional level. PCR analysis of CD95L mRNA in HepG2 and Hep3B cells is shown. Total RNA was extracted and RT-PCR was performed as described in Materials and Methods. (A) HepG2 or Hep3B cells were incubated with the indicated concentrations of 5-FU for 36 h. (B) HepG2 or Hep3B cells were incubated with 100 μg of 5-FU/ml for the indicated time periods. (C and D) 5-FU (100 μg/ml) was given to HepG2 cells for 48 h, and either the transcriptional inhibitor actinomycin C1(D) (C) or the translational inhibitor cycloheximide (D) was added at the indicated concentrations.