Figure 3.

CAR T-cell cytotoxicity can be modulated by epigenetic treatment. UCC and HBLAK cells were pretreated with 100 nM decitabine (white square), 3 nM romidepsin (orange circle), or DMSO (black rhombus) and subsequently co-cultured with CD19-, EGFR-, or CD44v6 CAR T-cells or untransduced (no virus) T-cells. After 16 h co-culture, lysis was determined by MTT assay. Graphs depict the mean values ( ± SD) for percentage of lysed cells from at least three independent experiments. Statistical significance (p-value <0.05) is denoted by asterisk and was determined by two-way ANOVA and Dunnett’s correction for multiple comparisons.