Fig. 6. Quantification of MDM2 activation in response to inhibition of p53:MDM2 interactions by immunofluorescence. (a), Confocal microscopy images of MDM2-immunofluorescence in SJSA-1 cells treated with inhibitor-peptides or NVP-CGM097 at the indicated concentrations. * = the fluorescent signal for the NVP-CGM097 positive control was brighter than for the other inhibitors and so the contrast was set two-fold lower. Scale bars 20 μm. (b), Quantification of nuclear MDM2 in the immunofluorescence experiment at different inhibitor concentrations. Shown are single values and mean ± SD, n = 3, ** = P < 0.005 in unpaired t-test.
