Table 1.

Oligonucleotides.

Oligo	Sequence 5' to 3'	Use
P1-pKD4-XhoI	ATATATCTCGAGTGTAGGCTGGAGCTGCTTCG, XhoI	PCR FRT-kan-FRT
P2-star-pKD4-XhoI	TATATACTCGAGCATATGAATATCCTCCTTAG, XhoI	PCR FRT-kan-FRT
O78pol-BamHI-F	ATATGGATCCATTGATCTCTATTATCGTATTATTCCAC, BamHI	PCR wzyO78
O78pol-EcoRI-R	ATATGAATTCGTTCGTAATGTGTCATTTCTCCATCGACAC, EcoRI	PCR wzyO78
O78 invers BglII-F	AATTAGATCTTTCCATTCGTTATTTGATAGC, BglII	Inverse PCR
O78 invers BglII-R	TTAAAGATCTAAGCGAATAACGCAGCAGCAAC, BglII	Inverse PCR
gne-SalI-F	ATCGGTCGACGCTCTCCCTTATGCG, SalI	PCR gne to pglJ
pglJ-SbfI-R	AACCACTTAACGTTTAACCTGCAGGATATAACGTGCAATTTTTACTTTATCAAAAGC, SbfI	PCR gne to pglJ
pglA-SbfI-F	AATTGCAATTTATATCCTGCAGGTTAAAATTTAAGGGTTGAAAATGAGAATAGG, SbfI	PCR pglA to pglG
pglG-BamHI-R	TCCTGTGGATCCCCCGGGCTGCAGGAATTCGATC, BamHI	PCR pglA to pglG
pglH-XhoI-F	ATATCTCGAGAGCTTAAAGAGGAGAAATGATGAAAATAAGC, XhoI	PCR pglH
pglK-XhoI-R	ATATCTCGAGATCATTTCTCCTCTTTAAGCTTACC, XhoI	PCR gne to pglK
gne-BamHI-F1	AATGGATCCAGTTTTTGCAAAAGCTTGTG, BamHI	PCR gne to pglA
pglA-BamHI-R	AATGGATCCTCATACATTCTTAATTACCC, BamHI	PCR gne to pglA
O78-outside-F	TCATAATATCATAACAGGGGTCATGAGC	PCR, confirm integration
O78-outside-R	TTGTGATTGTATAACCCAGCTCTTTATAC	PCR, confirm integration
pglA-F	AAATGCTGCCCAAGATGCTTTACAATACG	PCR, confirm integration
gne-R	TTAAAGTATGAGAACCTATATAACCTGC	PCR, confirm integration

Restriction sites are underlined; restriction enzymes (if applicable) are indicated.