Table 2.
Strains and plasmids.
| Strain | Genotype or relevant markers | Reference |
|---|---|---|
| Campylobacter | ||
| C. jejuni 81-176 | Clinical isolate, wild-type strain | Korlath et al., 1985 |
| E. coli | ||
| DH5α | F− endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG purB20 φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK−mK+), λ− | Invitrogen |
| APEC O:78(aroA) | Avian pathogenic E. coli, serotype O:78, mutation in aroA | Poulvac® E. coli |
| APEC O:78(aroA)/wzy::pgl::kan | APEC O:78(aroA) with pgl (gne-pglK-FRT-kan-FRT-pglHIJA) inserted into wzy locus, KanR | This study |
| Plasmid | ||
| pBluescript II KS(+) | Cloning vector, pUC ori, AmpR | Agilent |
| pACYC184 | Cloning vector, P15A ori, CmR, TetR | Chang and Cohen, 1978 |
| pACYC184pgl (ppgl) | Plasmid pACYC184 containing the 16kb C. jejuni pgl locus, CmR | Wacker et al., 2002 |
| ppgl ΔpglB | ppgl with a deletion of pglB, CmR | This study |
| ppgl ΔpglB-XhoI | ppgl ΔpglB with XhoI site between pglK and pglH, CmR | This study |
| ppgl-Kan | ppgl ΔpglB, XhoI with Kan cassette (flanked by FRT sites) inserted between pglK and pglH (via XhoI), CmR | This study |
| ppglgneK-kan-HIJA | PCR product containing gne-pglK-FRT-kan-FRT-pglHIJA inserted into pACYC184, CmR | This study |
| pBKS-wzyO78 | wzy (rfc) of APEC O78 inserted into pBluescript II KS(+), AmpR | This study |
| pBKS-wzyO78, BglII | pBKS-wzyO78 with BglII sites inserted into wzy, by inverse PCR | This study |
| pBKS-wzyO78-pgl-kanFRT | pBKS-wzyO78 with PCR product gne-pglK-FRT-kan-FRT-pglHIJA inserted into BglII site of wzyO78, AmpR | This study |
| pKD4 | FRT flanked kan cassette, template plasmid for PCR, KanR, AmpR | Datsenko and Wanner, 2000 |
| pKD46 | λ Red recombinase (γ, β, and exo from λ phage), araC-ParaB, AmpR | Datsenko and Wanner, 2000 |