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. 2021 Nov 17;8:788655. doi: 10.3389/fcvm.2021.788655

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Copyright © 2021 Uchikado, Ikeda, Sasaki, Iwabayashi, Akasaki and Ohishi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mdivi-1 treatment retarded excessive mitochondrial fission, ROS production, mitochondrial dysfunction and cellular senescence, which were induced by ox-LDL. (A) Representative images and quantitative analysis of Mito-tracker Red in ox-LDL-treated cells with mdivi-1 or the control vehicle. Scale bar = 10 μm. ^*P < 0.05 vs. ox-LDL with the control (n = 3 per group). (B) Electron microscopic images and quantitative analysis of mitochondria and average mitochondrial areas in ox-LDL-treated cells with mdivi-1 or the control. Scale bar = 1 μm. ^*P < 0.05 vs. ox-LDL with the control (n = 4 per group). (C) Immunoblots and quantitative analysis for Mfn1, Mfn2, Opa1, and β-actin in ox-LDL-treated cells with mdivi-1 or the control (n = 3 per group). (D) Representative images and quantitative analysis of SA-β gal staining of ox-LDL-treated cells with mdivi-1 or the control. ^**P < 0.01 vs. ox-LDL with the control (n = 9 per group). (E) Immunoblots and quantitative analysis for p53, p21, and β-actin in ox-LDL-treated cells with mdivi-1 or the control. ^*P < 0.05 vs. ox-LDL with the control (n = 3 per group). (F) Representative images of MitoSox Red in ox-LDL-treated cells with mdivi-1 or the control. Scale bar = 100 μm. (G) Representative images and quantitative analysis of JC-1 staining in ox-LDL-treated cells with mdivi-1 or the control. Scale bar = 100 μm. ^*P < 0.05 vs. ox-LDL with the control (n = 3 per group). All data are presented as the mean ± SEM. Statistical analysis were conducted by Wilcoxon rank sum test (A-C,E,G) and Student's t-test (D).