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Characterization of the anti-CGA monoclonal antibody. (A) Affinity result of the antibody. CA: cholic acid, CDCA: chenodeoxycholic acid, HSCA: hyodesoxycholic acid, and LCA: lithocholic acid; (B) cross-reactivity analysis of the antibody by indirect competitive enzyme-linked immunosorbent assay (ic-ELISA). (C) Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of the anti-GCA monoclonal antibody before and after purification.
