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. 2021 Nov 18;6(47):32005–32010. doi: 10.1021/acsomega.1c04779

Scheme 1. Schematic Illustration of GCA Bioprobe Preparation and Visually Enhanced Coagglutination Test of GCA,,,,,,,

Scheme 1

Splenocytes were isolated from the spleen of BALB/c mice immunized with a GCA antigen.

Hybridoma cells were obtained through the fusion of splenocytes and SP2/0 cells.

The target hybridoma cloned was acquired by further culturing and screening.

The target hybridoma cloned was injected into mice to collect ascites containing antibodies by intraperitoneal injection.

The anti-GCA monoclonal antibody was isolated and purified from ascites.

Staphylococcus aureus (S.aureus) turns red after CTC treatment.

A probe (mAb-S.aureus conjugate) is formed due to the combination of protein A on S.aureus and the Fc segment of the mAb.

When the probe and a sample containing GCA (probably forming a smaller cluster due to non-covalent interactions between GCA) is mixed on a glass slide, a larger red cluster is formed on the slide due to the recognition between the GCA cluster and anti-GCA, allowing us to detect the GCA by naked-eye visualization of the red spots.