Table 2. Shortlisted CTL Epitopes for the Final Vaccine Construction.
epitopes | protein | C-score | immunogenicity score | antigenicity | allergenicity | toxicity | epitope conservancy hit (maximum identity %) | human homology | interacting MHC-I allelesa |
---|---|---|---|---|---|---|---|---|---|
GSNDTELNY | L | 2.1551 | 0.13913 (positive) | 1.1545 (probable antigen) | probable non-allergen | non-toxin | 100 | non-homologue | HLA-A*01:01, HLA-B*58:01, HLA-A*30:02 |
WTIGNRAPY | L | 2.4674 | 0.14626 (positive) | 1.2809 (probable antigen) | probable non-allergen | non-toxin | 100 | non-homologue | HLA-A*26:01, HLA-A*30:02, HLA-A*01:01, HLA-C*03:03, HLA-B*15:01, HLA-B*35:01 |
FDERRHVAM | NP | 2.2254 | 0.22313 (positive) | 0.9841 (probable antigen) | probable non-allergen | non-toxin | 100 | non-homologue | HLA-B*14:02, HLA-B*08:01, HLA-C*07:02 |
RPMNRPTAL | NP | 1.9018 | 0.03207 (positive) | 0.5629 (probable antigen) | probable non-allergen | non-toxin | 100 | non-homologue | HLA-B*07:02, HLA-B*08:01, HLA-B*39:01, HLA-B*48:01, HLA-B*14:02, HLA-B*53:01, HLA-B*35:01 |
NPHAQGIAL | GP | 1.5028 | 0.10415 (positive) | 1.0162 (probable antigen) | probable non-allergen | non-toxin | 100 | non-homologue | HLA-B*39:01, HLA-B*35:01, HLA-B*15:02, HLA-B*07:02 |
FTYNLTNLV | VP40 | 1.2927 | 0.00609 (positive) | 0.7288 (probable antigen) | probable non-allergen | non-toxin | 100 | non-homologue | HLA-A*02:01, HLA-A*68:02, HLA-A*02:06, HLA-A*01:01, HLA-A*26:01, HLA-B*51:01, HLA-C*06:02, HLA-C*15:02, HLA-B*46:01 |
RTTAPAAAF | VP35 | 1.4688 | 0.13388 (positive) | 0.4463 (probable antigen) | probable non-allergen | non-toxin | 100 | non-homologue | HLA-A*32:01, HLA-B*58:01, HLA-B*57:01, HLA-B*07:02, HLA-C*03:03, HLA-B*15:01, HLA-B*35:01 |
SLTCAGIRK | VP30 | 1.1697 | 0.17091 (positive) | 1.3530 (probable antigen) | probable non-allergen | non-toxin | 100 | non-homologue | HLA-A*03:01, HLA-A*11:01 |
ISPNLLGIY | VP24 | 1.4959 | 0.07501 (positive) | 1.0528 (probable antigen) | probable non-allergen | non-toxin | 100 | non-homologue | HLA-A*30:02, HLA-A*01:01, HLA-A*26:01, HLA-B*46:01 |
MHC-I alleles with percentile rank ≤2 were taken.