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. 2021 Nov 18;6(47):32043–32071. doi: 10.1021/acsomega.1c04817

Table 6. Physicochemical, Antigenic, and Allergenic Characteristics of the Vaccine Construct.

characteristics findings remarks
number of amino acids 542 suitable
molecular weight 58306.34 Da average
theoretical pI 9.56 basic
chemical formula C2632H4161N721O743S16  
extinction coefficient (at 280 nm in H2O) 50,100 M–1 cm–1  
estimated half-life (mammalian reticulocytes, in vitro) 30 h satisfactory
estimated half-life (yeast cells, in vivo) >20 h satisfactory
estimated half-life (E. coli, in vivo) >10 h satisfactory
instability index of vaccine 33.08 stable
aliphatic index of vaccine 77.34 thermostable
grand average of hydropathicity (GRAVY) –0.303 hydrophilic
antigenicity 0.7130 (VaxiJen v2.0) 0.762192 (ANTIGENPro) antigenic
allergenicity no (AllerTop v2.0) No (AllergenFP v1.0) No (AlgPred) non-allergen
solubility 0.928259 (SolPro) 0.509 (Protein-Sol) soluble
TM helices no suitable
signal peptides no suitable