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. Author manuscript; available in PMC: 2021 Dec 2.
Published in final edited form as: Cell Stem Cell. 2015 Aug 20;17(3):360–372. doi: 10.1016/j.stem.2015.07.013

Figure 1. Single-cell transcriptomes of adult neural stem cells and their immediate progeny.

Figure 1.

(A) A schematic diagram of the process of adult neurogenesis in the dentate gyrus of the mouse hippocampus. Once quiescent neural stem cells (qNSCs) become activated (aNSCs), they enter cell cycle and generate early intermediate progenitor cells (eIPCs), which in turn give rise to neuroblasts (NB), immature neurons (IN) and then mature neurons (MN). Area highlighted with blue background indicates cell types fluorescently labeled in adult Nestin-CFPnuc animals.

(B) Expression levels of transcript encoding CFP in each single cell and diluted whole dentate RNA samples (TPM, transcripts per million). Inset: enlarged view of CFP transcript levels in logarithmic scale of samples with low abundance of CFP transcript.

(C) Representative coverage profile of diluted total RNA from the whole dentate gyrus, CFP– individual cells, and CFP+ individual cells at selected genomic loci, including house-keeping genes (β-actin/Actb, Gapdh, ubiquitin B/Ubb), known NSC markers (Blbp/Fabp7, Gfap, Sox2), known IPC markers (Sox11, Tbr2/Eomes), and potential new NPC markers (Gstm1).

See also Figure S1.