Figure 1.

Stickers-and-spacers framework that can be used to describe Velo1^PLD. (A) The stickers-and-spacers framework subdivides biomolecules into sticker regions and spacer regions, whereby stickers contribute attractive interactions that drive phase transitions through multivalent interactions. (B) The Velo1 sequence architecture contains an N-terminal prion-like domain (Velo1^PLD) and four fragments (F1–F4) as originally defined by Böke et al.³⁹ The Velo1^PLD sequence is shown explicitly with aromatic residues colored orange and all other residues colored black. (C) Questions of interest in this study are how sticker clustering (left) and spacer-related interactions (right) can alter the formation and equilibrium state of condensates formed by PLDs.