Skip to main content
. 2021 Nov 15;60(47):3621–3632. doi: 10.1021/acs.biochem.1c00672

Figure 3.

Figure 3

Product inhibition patterns by SAM using assay 1. (A) ATP was varied (30–500 μM) at a fixed saturating concentration of l-Met (600 μM) and at the indicated concentrations of SAM. (B) l-Met was varied (5–45 μM) at a fixed saturating concentration of ATP (500 μM) and at the indicated concentrations of SAM. (C) ATP was varied (20–180 μM) at a fixed nonsaturating concentration of l-Met (20 μM) and the indicated concentrations of SAM. (D) l-Met was varied (10–45 μM) at a fixed nonsaturating concentration of ATP (70 μM) and the indicated concentrations of SAM. Symbols show experimental data, and solid lines show fits of all data points to eq 4 in panel A and eq 3 in panels B, C, and D.