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Inhibition patterns by the methionine analogue l-cLeu using assay 1. (A) l-Met was varied (6–600 μM) at a fixed saturating concentration of ATP (1 mM) and at the indicated concentrations of l-cLeu. (B) ATP was varied (45–500 μM) at a fixed nonsaturating l-Met concentration (20 μM) and at the indicated concentrations of l-cLeu. Symbols show experimental data, and solid lines show simultaneous fits of all data points to eq 2 in panel A and eq 4 in panel B.
