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. 2021 Nov 15;60(47):3621–3632. doi: 10.1021/acs.biochem.1c00672

Figure 5.

Figure 5

ITC thermograms of l-Met, ATP, and l-cLeu (± ATP) titrated into Mat2A. (A) l-Met (20 mM) titrated into Mat2A (87 μM). (B) ATP (2 mM) titrated into Mat2A (87 μM). (C) l-cLeu (2 mM) titrated into Mat2A (205 μM). (D) l-cLeu (2 mM) + ATP (2 mM) titrated into Mat2A (205 μM) + ATP (2 mM). All titrations performed at 20 °C. Unprocessed thermograms (top panels) and the binding isotherm from the integrated thermogram fit to the One Set of Sites model using the MicroCal PEAQ-ITC analysis software (bottom panels). Circles indicate the integrated heat, and the curve represents the best fit. Data shown are representative of four independent experiments.