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. 2021 Nov 15;60(47):3621–3632. doi: 10.1021/acs.biochem.1c00672

Figure 8.

Figure 8

Mat2A activity is stabilized by Mat2B. (A) Mat2A activity remaining after preincubation of 60 nM Mat2A at 37 °C for 0, 1, 2.5, 5, 15, and 120 min in the absence and presence of 30 nM Mat2B. ATP concentration is 1.25 mM, and l-Met concentration is 200 μM. (B) Dependence of rate on l-Met concentration using 60 nM Mat2A or 60 nM Mat2A/30 nM Mat2B complex preincubated at 37 °C for 15 min. l-Met was varied (5–600 μM), and ATP is fixed at 1.25 mM. Symbols show experimental data, and the solid lines represent the best fits to eq 6 in panel A and eq 5 in panel B.