Table 1. Steady-State Kinetic Constants of Human Mat2Aa.
| Mat2A onlyb,c | Mat2A onlyd,e | Mat2A + Mat2Bb,c | |
|---|---|---|---|
| kcat (s–1) | 0.34 ± 0.06 | 0.34 ± 0.02 | 0.32 ± 0.03 |
| Km(ATP) (μM) | 50 ± 10 | 55 ± 10 | 50 ± 5 |
| Km(l-Met) (μM) | 5 ± 2 | 8 ± 2 | 4 ± 1 |
| Ki(ATP) (μM) | 349 ± 66 | ND | 183 ± 38 |
a
At pH 7.5 and 22 °C.
b
Coupled phosphate detection assay.
c
Values are averages from five independent experiments, and errors are the standard deviation.
d
LC–MS SAM detection assay.
e
Values are from a single experiment with three replicates, and errors are the standard error. ND = not determined.