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. 2021 Dec 2;19:255. doi: 10.1186/s12915-021-01189-9

Table 1.

The ten diel genes with the highest amplitudes (daily fold change) in females, males and male heads

Dataset Gene ID Gene description Daily fold change Phase (ZT) JTK BH.Q
Female Smp_072330 Heat shock protein 90 2.30 14.5 0.00032
Smp_326610 Trematode eggshell synthesis domain-containing protein 1.56 6.5 0.00191
Smp_004780 FKBP-type peptidylprolyl isomerase (PPIase) 1.41 16.5 0.00019
Smp_044850 Ribokinase 1.41 16.5 0.00770
Smp_342000 FMRFamide-activated amiloride-sensitive sodium channel 1.38 8.5 0.00191
Smp_069130 Heat shock protein 70 (Hsp70)-4 1.35 14.5 0.00029
Smp_319380 n/a 1.33 2.5 0.00362
Smp_244190 ZP domain-containing protein 1.31 14.5 0.00588
Smp_340010 Putative eggshell protein 1.30 4.5 0.00235
Smp_064860 Putative heat shock protein 70 (Hsp70)-interacting protein 1.30 16.5 7.92E−05
Male Smp_307450 SmKI-1, a BPTI/Kunitz protease inhibitor domain protein 1.83 4.5 0.00311
Smp_049550 Putative heat shock protein 70 (Hsp70) 1.62 16.5 1.21E−06
Smp_324960 Hypothetical protein 1.57 18.5 0.00112
Smp_327270 Very-long-chain 3-oxoacyl-CoA reductase 1.52 18.5 0.00763
Smp_327260 Transmembrane protein 45B 1.49 18.5 0.00468
Smp_013950 Solute carrier family 43 member 3 1.48 0.5 0.00112
Smp_328570 n/a 1.48 4.5 0.00763
Smp_327240 Hypothetical protein 1.45 18.5 0.00651
Smp_004780 FKBP-type Peptidylprolyl isomerase (PPIase) 1.36 16.5 7.31E−05
Smp_013790 Probable ATP-dependent RNA helicase, DDX5 1.35 0.5 0.00047
Male head Smp_049550 Putative heat shock protein 70 (Hsp70) 1.63 16.5 1.43E−05
Smp_004780 FKBP-type peptidylprolyl isomerase (PPIase) 1.35 16.5 0.00055
Smp_145560 Monocarboxylate transporter 9 1.33 2.5 0.00236
Smp_124820 Putative chromosome region maintenance protein 1/exportin 1.30 18.5 0.00236
Smp_069130 Heat shock protein 70 (Hsp70)-4 1.30 16.5 0.00236
Smp_333170 85/88 kDa calcium-independent phospholipase A2 1.28 18.5 0.00188
Smp_113620 Serine/arginine-rich splicing factor 2 1.27 16.5 0.00297
Smp_049600 Putative DNAj (Hsp40) homologue, subfamily C, member 3 1.25 16.5 7.31E−05
Smp_214080 VEZF1/Protein suppressor of hairy wing/Zld/ C2H2-type 1.25 16.5 7.49E−06
Smp_246230 Nicotinate-nucleotide pyrophosphorylase 1.24 16.5 0.00035

The ten diel genes with the greatest fold change in expression over a 24-h period were identified by ranking the diel genes by fold change in each dataset. Their daily fold change, peak phase of expression (zeitgeber time, ZT, bold = night) and significance of 24-h rhythmicity determined using JTK_Cycle are presented