Agarose gel (1%) electrophoresis of the PCR products amplified from DNA of BPC infected with rickettsiae derived from infected IDE8 cells (lane 3), uninfected IDE8 cells (lane 5), IDE8 cells infected with rickettsiae (lane 7), uninfected BPC (lane 9), BPC infected with C. ruminantium (Gardel) (lane 11), negative control distilled water (lane 13), and positive control C. ruminantium (Gardel) washed EB (lane 15). Molecular weight markers (lane 1) were a 1-kb ladder (Life Technologies).