Figure 6.

Injection and lysis of a plug of fluorescently-labeled endothelial cells. The schematic contains labels (A-D) that correspond to the location where the fluorescent micrographs were taken. Bovine pulmonary endothelial cells were incubated with acridine orange (intracellular dye) and added to the sample reservoir. As shown in A), cells were pumped into the device and, when analysis was desired, directed towards the injection waste by actuating the injection valves. The analysis channel contained a 50 mM solution of sodium dodecyl sulfate (SDS) and when a plug of cells where injected into the channel, they started to lyse (B and C). Complete lysis occurred ^~22 sec after the injection process was initiated (D).