Figure 6.

Functional responses to ethologically relevant sounds and corresponding distributions of layer 5 and layer 6 corticocollicular neurons in mouse AC. A, Time spectrograms of four classes of mouse calls used in this study. Calls 1 and 2 are low-frequency stress calls, call 3 is a restraint stress-induced medium-frequency call, and call 4 is a mating call. B, ΔF/F responses to the four classes of species-specific calls. C, D, A threshold level at 2.5 SDs was set to responses in A (C) to display the peaks of stimulus-evoked cortical activity (D). D, E, Distributions of layers 5 (D) and 6 (E) corticocollicular cells. F, G, Overlays of functional maps from C with layer 5 (F) and layer 6 (G) corticocollicular cells. Scale bar, 500 μm. Sound pressure level was ∼80 dB SPL for all calls. Sound pressure levels: 5 kHz (70 dB SPL), 10 kHz (30 dB SPL), 20 kHz (29 dB SPL), and 30 kHz (50 dB SPL). N = 10 repetitions of each stimulus. Mean responses are displayed.