FIGURE 3.

Drug response in 2D cells vs 3D bioprinted cells. (A) Response of Caco-2 cells grown in monolayers (2D) to different concentrations of irinotecan, 5-fluoruracil (5-FU), and oxaliplatin as annotated and a summary table of the respective IC₅₀ values. Cells were seeded and treated on the next day with the inhibitors for 72 h. Cell viability was measured with the MTT test, and IC₅₀ values were calculated from at least 2 biological replicates in technical triplicates. IC₅₀ values and 95% confidence interval values are both in ug/ml. (B) Response of 3D bioprinted cells (biological duplicates in technical duplicates) to 4 concentrations of the 3 inhibitors as annotated. Caco-2 cells were bioprinted (as described in Methods) and grown for 2 weeks prior to drug treatment. Error bars represent mean with SD. *p ≤ 0.05, **p ≤ 0.005, ***p < 0.0005, and ****p < 0.0001, ns: not significant, Student’s t-test. Asterisk symbols immediately above error bars indicate significance compared to control samples; lines with symbols show significance between the relevant treatment groups.