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. 2021 Nov 18;9:755563. doi: 10.3389/fbioe.2021.755563

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Copyright © 2021 Sbirkov, Molander, Milet, Bodurov, Atanasov, Penkov, Belev, Forraz, McGuckin and Sarafian.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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3D bioprinting of primary CRC samples. Representative pictures of 3D bioprinted primary CRC cells taken on day 14 after printing. (A) Live/dead staining with calcein AM (live cells: green fluorescence) and propidium iodide (dead cells: red fluorescence) under ×4 and ×10 magnification and relative quantification of percentage live cells (per 1 mm²). Error bars represent SD of the mean from at least two images. (B) Representative pictures of the hematoxylin and eosin (H&E) staining of sections from 3D bioprinted primary cells under ×4 and ×10 magnification.